Molecular Fluorescence Spectroscopy
- Introduction to the fluorescence spectroscopy principle
- Familiarization with the fluorescence Instrumentation
- Familiarization with Excitation and Emission Spectra, Mirror Image Relation and Stokes Shift
- Demonstration of Solvent Effects on Fluorescence Spectra of a Fluorophore
- Demonstration of pH Effect on Fluorescence Excitation and Emission Spectra of a Fluorophore
- Effects of Fluorophore Concentration on Fluorescence Spectra: Inner Filter Effects
- Relationship between the Concentration of a Fluorophore and its Fluorescence Intensity: Determination of Unknown Concentration of an Analyte
- Determination of Fluorescence Quantum Yield of a Fluorophore
- Fluorescence Quenching and Stern-Volmer Plot: Estimation of the Quenching Constant from Stern-Volmer Plot
- Effects of Excimer and Exciplex Formations on Fluorescence Emission